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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene has actually been noticed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a essential function in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants have been documented but in-depth nucleotide sequence information and facts just isn't nevertheless offered.

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Blocking peptides are peptides that bind specially towards the goal antibody and block antibody binding. These peptide normally contains the epitope acknowledged through the antibody.

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This guideline outlines additional feasible optimization of mobile and immunoassay parameters to be certain the best possible results are...

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This information outlines even further doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...

Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is unique.

Concentrate on Facts Cyclin E belongs to the hugely conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance from the cell cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is degraded as cells development via S section.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...

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